KUMJ | VOL. 18 | NO. 1 | ISSUE 69 | JAN.-MARCH, 2020
Multi-drug Resistant and Extended Spectrum β-lactamase Producing Salmonella Species Isolated from Fresh Chicken Liver Samples
Adhikari S, Khadka S, Sapkota S, Sharma BR, Ghimire A, Chalise M, Gurung D, Kunwar S
Abstract: Background
Emergence of antibiotic resistance among microbes contaminating the fresh meat
products is a global public health concern as they can be easily transmitted to
humans through their consumption and contact.
Objective
The current study was conducted to determine the distribution of antimicrobial
resistance among Salmonella species isolated from fresh chicken liver samples with
special emphasis on extended spectrum beta-lactamase (ESBL) production.
Method
A total of 200 fresh chicken liver samples were cultivated for the isolation of
Salmonella and further subcultivated to detect extended spectrum beta-lactamase
production among them. Antimicrobial susceptibility testing (AST) was done by disk
diffusion method using a panel of 7 antimicrobials.
Result
Out of 200 samples analyzed, 61 (30.5%) samples harbored Salmonella species out
of which 15 (7.5%) samples showed the presence of Salmonella Typhi. A significant
association was noted in the incidence of Salmonella with various factors pertaining to
the butchers, such as age, sex, literacy rate, practices of washing knives and chopping
board, wearing aprons and gloves and type of water used (p < 0.05). Salmonella
isolates were highly sensitive to amikacin (82.0%) and least sensitive to tetracycline
(3.3%). All the isolates were resistant to colistin. Sixty (98.4%) isolates were identified
as multi-drug resistant (MDR). The total number of extended spectrum betalactamase
producers
reported
among Salmonella
isolates
was
29 (47.5%).
Conclusion
The results indicate that the fresh chicken liver samples sold in Bharatpur Metropolis
are reservoirs of multi-drug resistant Salmonella, including extended spectrum betalactamase
producers,
that
could
potentially
be
transmitted
to
the
humans
by
direct
contact
or through
inadequate
cooking.
Keyword : Antimicrobial susceptibility testing, Extended spectrum beta-lactamase, Fresh chicken liver samples, Multi-drug resistant, Salmonella
